retronectin (TaKaRa)
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TaKaRa
retronectin
Retronectin, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+fibronectin+fragment/RetroNectin+Recombinant+Human+Fibronectin+Fragment/pmc13195874-100-9-10
Average 97 stars, based on 1120 article reviews
Retronectin, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+fibronectin+fragment/RetroNectin+Recombinant+Human+Fibronectin+Fragment/pmc13195874-100-9-10
Average 97 stars, based on 1120 article reviews
retronectin - by Bioz Stars,
2026-09
97/100 stars
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Transduction:Article Title: MUC1-targeted CAR-T cell secreted anti-PD-1 IgG antibody enhances antitumor activity in Cholangiocarcinoma. Article Snippet: .. For T cell transduction, the retroviral supernatant was added to a 24-well nontissue culture-treated plate coated with Article Title: RMzyme: regulations of RNA-modifying enzymes in humans. Article Snippet: .. For HL60 leukemia cells, transduction with pLV3-CMV-ALKBH5-3×FLAG-CopGFP-puro viral supernatant was performed as follows: six-well plates were coated with 10 μg/mL Article Title: CD147 chimeric antigen receptors and methods of use Article Snippet: .. NK-92MI cells were transduced with retroviral supernatants on day 3 in plates coated with Article Title: RMzyme: regulations of RNA-modifying enzymes in humans Article Snippet: The cells were cultured in SFEM (09650, STEMCELL) supplemented with 20% fetal bovine serum (FBS), 1% penicillin–streptomycin, and the following recombinant human cytokines: stem cell factor (rhSCF, 100 ng/mL, 250--03, PeproTech), thrombopoietin (rhTPO, 10 ng/mL, 300--18, PeproTech), Flt-3 ligand (rhFlt-3 L, 10 ng/mL, 300--19, PeproTech), interleukin-3 (rhIL-3, 10 ng/mL, 200--03, PeproTech), and interleukin-6 (rhIL-6, 10 ng/mL, 200--06, PeproTech). .. For HL60 leukemia cells, transduction with pLV3-CMV-ALKBH5-3×FLAG-CopGFP-puro viral supernatant was performed as follows: six-well plates were coated with 10 μg/mL Retroviral:Article Title: MUC1-targeted CAR-T cell secreted anti-PD-1 IgG antibody enhances antitumor activity in Cholangiocarcinoma. Article Snippet: .. For T cell transduction, the retroviral supernatant was added to a 24-well nontissue culture-treated plate coated with Article Title: IL-7 armed binary CAR T cell strategy to augment potency against solid tumors Article Snippet: Cells were cultured in complete medium (RPMI-1640 containing 45% Clicks medium (Irvine Scientific, Inc., Santa Ana, CA), 10% FBS and 2 mM L-GlutaMAX), which was supplemented with recombinant human IL-2 (50 U/mL, NIH, Bethesda, VA) on day 1. .. On day 3 post OKT3/CD28 T blast generation, 1 mL of retroviral supernatant was added to a 24-well non-tissue culture-treated plate pre-coated with Article Title: CD147 chimeric antigen receptors and methods of use Article Snippet: .. NK-92MI cells were transduced with retroviral supernatants on day 3 in plates coated with Article Title: IL-7 armed binary CAR T cell strategy to augment potency against solid tumors Article Snippet: Cells were cultured in complete medium (RPMI-1640 containing 45% Clicks medium (Irvine Scientific, Inc., Santa Ana, CA), 10% FBS and 2 mM L-GlutaMAX), which was supplemented with recombinant human IL-2 (50 U/mL, NIH, Bethesda, VA) on day 1. .. On day 3 post OKT3/CD28 T blast generation, 1 mL of retroviral supernatant was added to a 24-well non-tissue culture-treated plate pre-coated with Recombinant:Article Title: MUC1-targeted CAR-T cell secreted anti-PD-1 IgG antibody enhances antitumor activity in Cholangiocarcinoma. Article Snippet: .. For T cell transduction, the retroviral supernatant was added to a 24-well nontissue culture-treated plate coated with Article Title: RMzyme: regulations of RNA-modifying enzymes in humans. Article Snippet: .. For HL60 leukemia cells, transduction with pLV3-CMV-ALKBH5-3×FLAG-CopGFP-puro viral supernatant was performed as follows: six-well plates were coated with 10 μg/mL Article Title: IL-7 armed binary CAR T cell strategy to augment potency against solid tumors Article Snippet: Cells were cultured in complete medium (RPMI-1640 containing 45% Clicks medium (Irvine Scientific, Inc., Santa Ana, CA), 10% FBS and 2 mM L-GlutaMAX), which was supplemented with recombinant human IL-2 (50 U/mL, NIH, Bethesda, VA) on day 1. .. On day 3 post OKT3/CD28 T blast generation, 1 mL of retroviral supernatant was added to a 24-well non-tissue culture-treated plate pre-coated with Article Title: CD147 chimeric antigen receptors and methods of use Article Snippet: .. NK-92MI cells were transduced with retroviral supernatants on day 3 in plates coated with Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T Article Snippet: The anti-CCL2/MCP-1 antibody (A7277) was purchased from ABclonal (Wuhan, China). .. The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T. Article Snippet: The anti-CCL2/MCP-1 antibody (A7277) was purchased from ABclonal (Wuhan, China). .. The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the Article Title: IL-7 armed binary CAR T cell strategy to augment potency against solid tumors Article Snippet: Cells were cultured in complete medium (RPMI-1640 containing 45% Clicks medium (Irvine Scientific, Inc., Santa Ana, CA), 10% FBS and 2 mM L-GlutaMAX), which was supplemented with recombinant human IL-2 (50 U/mL, NIH, Bethesda, VA) on day 1. .. On day 3 post OKT3/CD28 T blast generation, 1 mL of retroviral supernatant was added to a 24-well non-tissue culture-treated plate pre-coated with Article Title: RMzyme: regulations of RNA-modifying enzymes in humans Article Snippet: The cells were cultured in SFEM (09650, STEMCELL) supplemented with 20% fetal bovine serum (FBS), 1% penicillin–streptomycin, and the following recombinant human cytokines: stem cell factor (rhSCF, 100 ng/mL, 250--03, PeproTech), thrombopoietin (rhTPO, 10 ng/mL, 300--18, PeproTech), Flt-3 ligand (rhFlt-3 L, 10 ng/mL, 300--19, PeproTech), interleukin-3 (rhIL-3, 10 ng/mL, 200--03, PeproTech), and interleukin-6 (rhIL-6, 10 ng/mL, 200--06, PeproTech). .. For HL60 leukemia cells, transduction with pLV3-CMV-ALKBH5-3×FLAG-CopGFP-puro viral supernatant was performed as follows: six-well plates were coated with 10 μg/mL Centrifugation:Article Title: MUC1-targeted CAR-T cell secreted anti-PD-1 IgG antibody enhances antitumor activity in Cholangiocarcinoma. Article Snippet: .. For T cell transduction, the retroviral supernatant was added to a 24-well nontissue culture-treated plate coated with Cell Culture:Article Title: MUC1-targeted CAR-T cell secreted anti-PD-1 IgG antibody enhances antitumor activity in Cholangiocarcinoma. Article Snippet: .. For T cell transduction, the retroviral supernatant was added to a 24-well nontissue culture-treated plate coated with Isolation:Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T Article Snippet: The anti-CCL2/MCP-1 antibody (A7277) was purchased from ABclonal (Wuhan, China). .. The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T. Article Snippet: The anti-CCL2/MCP-1 antibody (A7277) was purchased from ABclonal (Wuhan, China). .. The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the Transfection:Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T Article Snippet: The anti-CCL2/MCP-1 antibody (A7277) was purchased from ABclonal (Wuhan, China). .. The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T. Article Snippet: The anti-CCL2/MCP-1 antibody (A7277) was purchased from ABclonal (Wuhan, China). .. The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the Cytotoxicity Assay:Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T Article Snippet: The anti-CCL2/MCP-1 antibody (A7277) was purchased from ABclonal (Wuhan, China). .. The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T. Article Snippet: The anti-CCL2/MCP-1 antibody (A7277) was purchased from ABclonal (Wuhan, China). .. The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the |